In-vitro Shoot Proliferation of Calotropis Procera

Calotropis procera, a plant of family Asclepiadaceae is of immense importance as multipurpose plant with medicinal as well as traditional uses. Latex of this plant has been used to cure disease like leprosy, syphilis, inflammation, cutaneous infections, and malarial fever and in many more. In vitro culture of this pharmacologically plant is required otherwise it leads the segregated loss of desirable traits. A comparative study of this plant has come out through in vitro culture of nodal segment of the mature plant. The explants were inoculated in MS medium fortified with different plant growth regulators alone or in combinations. Maximum shoot proliferation was observed i.e. 4 shoots per explants on 5+1 mg/L of BAP+NAA within 30 days of inoculation. Maximum shoot length was 1.41±0.0141 cm on 2+1 mg/L of BAP+NAA within 20 days after inoculation.